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  5. 3M™ Bovine Casein Protein ELISA Kit E96CAS, 96 wells per kit

3M™ Bovine Casein Protein ELISA Kit E96CAS, 96 wells per kit

3M ID 7100151339UPC 00707387784959
  • Designed to detect both processed and unprocessed target allergen proteins
  • Test can accommodate environmental swab, CIP rinse water and food samples
  • A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time
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Specifications
Allergen
Bovine Casein
Brand
3M™
Limit of Quantification (LOQ)
0.5 ppm
Manufacturing Segment
Environmental Monitoring, Packaging & Storage Testing, Raw Milk Reception, Ready-to-eat
Product Code
E96CAS
Product Type
Test
Protein Threshold
0.5 ppm
Recommended Industry
Bakery, Beverage & Bottled Water, Chemicals, Confectionary, Dairy, Eggs, Food Services & Catering, Fruits & Vegetables, Ingredients, Meat, Nutraceuticals, Nuts, Pet Food & Animal Feed, Poultry, Prepared & Processed Foods, Reference Labs, Sauces & Spices, Seafood
Storage Temperature
36-46°F
Storage Temperature (Celsius)
2-8°C
Test Time
50 min
Test Type
Allergen
Details
  • Designed to detect both processed and unprocessed target allergen proteins
  • Test can accommodate environmental swab, CIP rinse water and food samples
  • A universal extraction protocol streamlines testing for multiple analytes, saving you reagents and valuable time

The 3M™ Bovine Casein Protein ELISA Kit is intended for the detection of bovine casein proteins in clean-in-place water (CIP) final rinse water, environmental swab samples, food ingredients, and processed food products.

The 3M™ Bovine Casein Protein ELISA Kit utilizes a sandwich ELISA. The bovine casein proteins present in the sample react with the anti-bovine casein antibody, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-bovine casein antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound bovine casein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The colour development from this enzymatic reaction varies directly with the concentration of bovine casein protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of bovine casein protein in the test sample. The quantity of bovine casein protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.

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